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naglers reaction

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naglers reaction

Post  sakia_fazal on Sat Jun 06, 2009 10:53 pm

Naglers reaction: When clostridium perfringes is grown on a medium containing 6% agar, 5% fildes peptic digest of blood and 20% human serum with the anti toxic spread on one half of the plate,after the growth of the colonies you will observe that there is a zone of opacity in the half without the antitoxin.There will no opacity in the other half due to nuetralization of the alpha toxin.It is a specific lecithinase effect daignostic of clostridium perfinges in clinical specimen.

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