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Lab diagnosis of Kala-azar

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Lab diagnosis of Kala-azar

Post  sudheerkher on Fri Nov 14, 2008 8:30 pm

Lara Motamedi 2006M029

Lab diagnosis of Kala-azar


Direct evidence of diagnosis of kala-azar is:
• Identification of amastigotes in giemsa stained tissue/fluid.
• Blood culture for identification of promastigote
• Biopsy of lymph node, bone marrow, splenic puncture.

Indirect evidence of diagnosis of kala- azar is-
• Blood count to indicate progressive leukopenia.
• Serum tests with :
1. ELISA
2. Indirect fluorescent antibodies
3. Complement fixation- positive within 3 weeks
4. Napier test- positive after 3 months
5. Chopra’s test- less reliable

Medium used for culture is blood agar Nicolle-Novy-MacNeal (NNN) media.

Bone marrow smear showing LD body:

sudheerkher

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